GAMT Rabbit Polyclonal Antibody
cat.: HA500071
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GAMT aa 1-222. |
| Positive control: | Hela cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HeLa. |
| Subcellular location: | Cytosol,nucleus,cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:100 |
| Uniprot #: | SwissProt: Q14353 Human | O35969 Mouse | P10868 Rat |
| Alternative names: | CCDS2 Epididymis secretory protein Li 20 GAMT GAMT_HUMAN Guanidinoacetate N methyltransferase Guanidinoacetate N-methyltransferase HEL-S-20 PIG2 TP53I2 |
Images
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Fig1:
Western blot analysis of GAMT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500071, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling GAMT with Rabbit anti-GAMT antibody (HA500071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GAMT antibody (HA500071) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.