| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 22 kDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human CD69 |
| Positive control: | K562 cell lysate, mouse bone marrow tissue lysate, mouse spleen tissue lysate, HL-60 cell lysate, rat bone marrow tissue lysates. |
| Subcellular location: | Membrane; Single-pass type II membrane protein. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q07108 Human | P37217 Mouse |
| Alternative names: | Activation inducer molecule (AIM/CD69) Activation inducer molecule AIM BL-AC/P26 BLAC/P26 C-type lectin domain family 2 member C CD69 CD69 antigen (p60, early T-cell activation antigen) CD69 antigen CD69 molecule CD69_HUMAN CLEC2C EA1 Early activation antigen CD69 Early lymphocyte activation antigen Early T cell activation antigen p60 Early T-cell activation antigen p60 GP32/28 Leu23 Leukocyte surface antigen Leu-23 MLR-3 MLR3 VEA Very Early Activation Antigen |
|
Fig1: Daudi (H) treated with PMA (50 ng/ml) for 24 h, 25 μg total protein per lane of cell lysates (see on figure) probed with CD69 polyclonal antibody, unconjugated (HA500073) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. |