CD69 Rabbit Polyclonal Antibody
cat.: HA500073
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD69 aa 1-199. |
| Positive control: | K562 cell lysate, mouse bone marrow tissue lysate, mouse spleen tissue lysate, HL-60 cell lysate, rat bone marrow tissue lysates. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q07108 Human | P37217 Mouse |
| Alternative names: | Activation inducer molecule (AIM/CD69) Activation inducer molecule AIM BL-AC/P26 BLAC/P26 C-type lectin domain family 2 member C CD69 CD69 antigen (p60, early T-cell activation antigen) CD69 antigen CD69 molecule CD69_HUMAN CLEC2C EA1 Early activation antigen CD69 Early lymphocyte activation antigen Early T cell activation antigen p60 Early T-cell activation antigen p60 GP32/28 Leu23 Leukocyte surface antigen Leu-23 MLR-3 MLR3 VEA Very Early Activation Antigen |
Images
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Fig1:
Western blot analysis of CD69 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500073, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Mouse bone marrow tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: HL-60 cell lysate |
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Fig2: Western blot analysis of CD69 on rat bone marrow tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500073, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.