PPP1CA Rabbit Polyclonal Antibody
cat.: HA500074
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PPP1CA aa 291-330. |
| Positive control: | MCF-7 cell lysate, A549 cell lysate, NIH/3T3 cell lysat,SK-OV-3 cell lysate, rat brain tissue lysate, mouse brain tissue lysate, rat large intestine tissue, mouse kidney tissue. |
| Subcellular location: | Nucleus, nucleoplasm, nucleolus, cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P62136 Human | P62137 Mouse | P62138 Rat |
| Alternative names: | Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit Catalytic subunit EC 3.1.3.16 MGC15877 MGC1674 PP 1A PP-1A PP1A PP1A_HUMAN PP1alpha PP2C ALPHA PP2CA Ppp1ca Protein Phosphatase 2C Alpha Isoform Serine threonine protein phosphatase PP1 alpha catalytic subunit Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 Serine/threonine-protein phosphatase PP1-alpha catalytic subunit |
Images
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Fig1:
Western blot analysis of PPP1CA on different lysates with Rabbit anti-PPP1CA antibody (HA500074) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Lane 3: SK-OV-3 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: mouse brain tissue lysate (30 µg/Lane) Lane 6: rat brain tissue lysate (30 µg/Lane) Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500074, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 38 kDa Observed band size: 38 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-PPP1CA antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500074, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PPP1CA antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500074, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.