RALA Rabbit Polyclonal Antibody
cat.: HA500075
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 24 kDa
Isotype: IgG
Immunogen: Recombinant full length protein of human RALA.
Positive control: HepG2 cell lysate, Jurkat cell lysate, Hela cell lysate, mouse liver tissue lysate, rat thymus tissue lysate, A431, F9, rat brain tissue, mouse brain tissue.
Subcellular location: Cell membrane, Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:1,000-1:5,000
1:50-1:100
1:100-1:500
Uniprot #: SwissProt: P11233 Human | P63321 Mouse | P63322 Rat
Alternative names: MGC48949 Ral a Ral A protein RAL RALA RALA_HUMAN Ras family small GTP binding protein RALA RAS like protein A Ras related protein RalA Ras-related protein Ral-A v ral simian leukemia viral oncogene homolog A (ras related) v ral simian leukemia viral oncogene homolog A
Images
HA500075_1.jpg Fig1: Western blot analysis of RALA on different lysates with Rabbit anti-RALA antibody (HA500075) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-RALA KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 24 kDa
Observed band size: 24 kDa

Exposure time: 12 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500075) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA500075_2.jpg Fig2: Western blot analysis of RALA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500075, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Rat thymus tissue lysate
HA500075_3.jpg Fig3: ICC staining of RALA in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500075, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500075_4.jpg Fig4: ICC staining of RALA in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500075, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500075_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RALA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500075, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500075_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-RALA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500075, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.