HOXA9 Rabbit Polyclonal Antibody
cat.: HA500077
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human HOXA9 aa 20-60. |
| Positive control: | 293T (Human embryonic kidney cell) cell lysate, mouse kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: P31269 Human | P09631 Mouse |
| Alternative names: | ABD-B Abd-B, drosophila, homolog of D6a9 Homeobox 1G Homeobox A9 Homeobox protein Hox-1.7 Homeobox protein Hox-1G Homeobox protein Hox-A9 Homeodomain protein HOXA9 Hox-1.7 Hox-1.7, mouse, homolog of HOX1 Hox1.7 Hox1G Hox1r5 Hoxa-9 Hoxa7 HOXA9 HOXA9/MSI2 fusion gene, included HOXA9/NUP98 fusion gene, included HXA9_HUMAN MGC1934 |
Images
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Fig1:
Western blot analysis of HOXA9 on different lysates with Rabbit anti-HOXA9 antibody (HA500077) at 1/500 dilution. Lane 1: 293T (Human embryonic kidney cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500077, 1/500 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/40,000 in 5% NFDM/TBST, 1 hour at room temperature |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HOXA9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500077, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.