HOXA9 Rabbit Polyclonal Antibody
cat.: HA500077
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human HOXA9 aa 20-60. |
| Positive control: | HepG2 cell lysate, mouse kidney tissue lysate, mouse kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: P31269 Human | P09631 Mouse |
| Alternative names: | ABD-B Abd-B, drosophila, homolog of D6a9 Homeobox 1G Homeobox A9 Homeobox protein Hox-1.7 Homeobox protein Hox-1G Homeobox protein Hox-A9 Homeodomain protein HOXA9 Hox-1.7 Hox-1.7, mouse, homolog of HOX1 Hox1.7 Hox1G Hox1r5 Hoxa-9 Hoxa7 HOXA9 HOXA9/MSI2 fusion gene, included HOXA9/NUP98 fusion gene, included HXA9_HUMAN MGC1934 |
Images
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Fig1:
Western blot analysis of HOXA9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500077, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Mouse kidney tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HOXA9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500077, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.