| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 12 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human EG-VEGF aa 41-86. |
| Positive control: | MCF-7 cell lysate, mouse kidney tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P58294 Human | Q14A28 Mouse |
| Alternative names: | Black mamba toxin related protein EG VEGF EG-VEGF EGVEGF Endocrine-gland-derived vascular endothelial growth factor Mambakine PK1 PRK1 PROK1 PROK1_HUMAN Prokineticin 1 Prokineticin-1 |
|
Fig1:
Western blot analysis of EG-VEGF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500078, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 1: Mouse kidney tissue lysate |