| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 113 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NR3B aa 179-220 (Extracellular domain). |
| Positive control: | THP-1 cell lysate, 293 cell lysate, Daudi cell lysate. |
| Subcellular location: | Cell membrane, postsynaptic cell membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: O60391 Human |
| Alternative names: | GluN3B Glutamate [NMDA] receptor subunit 3B Glutamate receptor ionotropic N methyl D aspartate 3B Glutamate receptor ionotropic NMDA 3B GRIN3B N-methyl-D-aspartate receptor subtype 3B NMD3B_HUMAN NMDA receptor subunit 3B NMDA type glutamate receptor subunit NR3B NMDAR3B NR3B |
|
Fig1:
Western blot analysis of NR3B on THP-1 (Human acute monoblastic leukemia cell) lysates with Rabbit anti-NR3B antibody (HA500079) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 1 minutess; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500079, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 113 kDa Observed band size: 113 kDa |