Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 113 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human NR3B aa 179-220 (Extracellular domain). |
Positive control: | THP-1 cell lysate, 293 cell lysate, Daudi cell lysate. |
Subcellular location: | Cell membrane, postsynaptic cell membrane. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: O60391 Human |
Alternative names: | GluN3B Glutamate [NMDA] receptor subunit 3B Glutamate receptor ionotropic N methyl D aspartate 3B Glutamate receptor ionotropic NMDA 3B GRIN3B N-methyl-D-aspartate receptor subtype 3B NMD3B_HUMAN NMDA receptor subunit 3B NMDA type glutamate receptor subunit NR3B NMDAR3B NR3B |
Fig1:
Western blot analysis of NR3B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500079, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: 293 cell lysate Lane 2: Daudi cell lysate |