NR3B Rabbit Polyclonal Antibody
cat.: HA500079
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 113 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human NR3B aa 179-220 (Extracellular domain).
Positive control: THP-1 cell lysate, 293 cell lysate, Daudi cell lysate.
Subcellular location: Cell membrane, postsynaptic cell membrane.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: O60391 Human
Alternative names: GluN3B Glutamate [NMDA] receptor subunit 3B Glutamate receptor ionotropic N methyl D aspartate 3B Glutamate receptor ionotropic NMDA 3B GRIN3B N-methyl-D-aspartate receptor subtype 3B NMD3B_HUMAN NMDA receptor subunit 3B NMDA type glutamate receptor subunit NR3B NMDAR3B NR3B
Images
HA500079_1.jpg Fig1: Western blot analysis of NR3B on THP-1 (Human acute monoblastic leukemia cell) lysates with Rabbit anti-NR3B antibody (HA500079) at 1/5,000 dilution.

Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minutess; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500079, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 113 kDa
Observed band size: 113 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.