IL-5 Rabbit Polyclonal Antibody
cat.: HA500081
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, ELISA
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 15 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human IL-5 aa 60-110.
Positive control: LOVO cell lysates, LOVO cells.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  ELISA

1:500-1:1,000
1:50-1:200
1:5,000-1:10,000
Uniprot #: SwissProt: P05113 Human
Alternative names: B-cell differentiation factor I Colony stimulating factor EDF Eosinophil differentiation factor IL-5 IL5 IL5_HUMAN Interleukin 5 (colony stimulating factor, eosinophil) Interleukin 5 Interleukin-5 T Cell Replacing Factor T-cell replacing factor TRF
Images
HA500081_1.jpg Fig1: Western blot analysis of IL-5 on LOVO cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500081, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
HA500081_2.jpg Fig2: ICC staining of IL-5 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500081, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.