IL-5 Rabbit Polyclonal Antibody
cat.: HA500081
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL-5 aa 60-110. |
| Positive control: | LOVO cell lysates, LOVO cells. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell ELISA |
1:500-1:1,000 1:50-1:200 1:5,000-1:10,000 |
| Uniprot #: | SwissProt: P05113 Human |
| Alternative names: | B-cell differentiation factor I Colony stimulating factor EDF Eosinophil differentiation factor IL-5 IL5 IL5_HUMAN Interleukin 5 (colony stimulating factor, eosinophil) Interleukin 5 Interleukin-5 T Cell Replacing Factor T-cell replacing factor TRF |
Images
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Fig1: ICC staining of IL-5 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500081, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig2:
Western blot analysis of IL-5 on different lysates with Rabbit anti-IL-5 antibody (HA500081) at 1/1,000 dilution. Lane 1: LoVo (Human colorectal cancer cells) cell lysate Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500081, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 15.2 kDa Observed band size: 15 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.