HSD3B1 Rabbit Polyclonal Antibody
cat.: HA500082
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human HSD3B1 aa 320-373. |
| Positive control: | Human placenta tissue lysates, MCF-7, LOVO, human placenta tissue. |
| Subcellular location: | Endoplasmic reticulum, Membrane, Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000-1:5,000 1:50-1:200 1:100-1:500 |
| Uniprot #: | SwissProt: P14060 Human |
| Alternative names: | 3 beta and steroid delta isomerase 1 3 beta and steroid hydroxy delta 5 steroid dehydrogenase 3 beta HSD I 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I 3-beta-HSD I 3-beta-hydroxy-5-ene steroid dehydrogenase 3BETAHSD 3BH 3BHS1_HUMAN 3BHSD Delta-5-3-ketosteroid isomerase HGNC:5217 HSD3B HSD3B1 HSDB3 HSDB3A Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1 Hydroxy delta 5 steroid dehydrogenase Progesterone reductase SDR11E1 Steroid Delta-isomerase trophoblast antigen Trophoblast antigen FDO161G |
Images
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Fig1: Western blot analysis of HSD3B1 on human placenta tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500082, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of HSD3B1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500082, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of HSD3B1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500082, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HSD3B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500082, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.