VGluT1 Rabbit Polyclonal Antibody
cat.: HA500083
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human VGluT1 aa 510-560. |
| Positive control: | Human brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysates, mouse testis tissue. |
| Subcellular location: | Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q9P2U7 Human | Q3TXX4 Mouse | Q62634 Rat |
| Alternative names: | BNPI Brain specific Na (+) dependent inorganic phosphate cotransporter Brain specific Na dependent inorganic phosphate cotransporter Brain-specific Na(+)-dependent inorganic phosphate cotransporter Slc17a7 Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 Solute carrier family 17 member 7 Vesicular glutamate transporter 1 VGLU1_HUMAN VGLUT 1 VGluT1 |
Images
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Fig1:
Western blot analysis of VGluT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500083, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human brain tissue lysate Lane 2: Mouse hippocampus tissue lysate |
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Fig2: Western blot analysis of VGluT1 on rat brain tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500083, 1/200,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-VGluT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500083, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.