Inhibin beta B Rabbit Polyclonal Antibody
cat.: HA500086
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Inhibin beta B aa 345-380. |
| Positive control: | SK-Br-3 cell lysate, MCF-7 cell lysate, human uterus tissue, mouse brain tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P09529 Human | Q04999 Mouse |
| Alternative names: | Activin beta B chain Activin beta-B chain INHBB INHBB_HUMAN Inhibin beta 2 Inhibin beta B chain Inhibin beta B subunit |
Images
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Fig1:
Western blot analysis of Inhibin beta B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500086, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: MCF-7 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Inhibin beta B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500086, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Inhibin beta B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500086, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.