Ly6c Rabbit Polyclonal Antibody
cat.: HA500087
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Ly6c2 aa 45-77. |
| Positive control: | C2C12 cell lysate, Mouse lung tissue lysate, Mouse spleen tissue lysate, Mouse kidney tissue lysate, mouse heart tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: P0CW02 Mouse | P0CW03 Mouse |
| Alternative names: | Ly 6c Ly6c protein Ly6c1 Ly6c2 Lymphocyte antigen 6 complex Lymphocyte antigen 6 complex locus C Lymphocyte antigen 6C Lymphocyte antigen 6C1 Lymphocyte antigen 6C2 Lymphocyte antigen Ly 6C |
Images
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Fig1:
Western blot analysis of Ly6c on different lysates with Rabbit anti-Ly6c antibody (HA500087) at 1/2,000 dilution. Lane 1: C2C12 cell lysate Lane 2: Mouse lung tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Mouse kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 1 minute 50 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500087) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Ly6c antibody (HA500087) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500087) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.