Ly6c Rabbit Polyclonal Antibody
cat.: HA500088
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Ly6c1 aa 75-100. |
| Positive control: | Mouse lung tissue lysate, mouse spleen tissue lysate, mouse testis tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate, mouse brain tissue lysate, mouse lung tissue, mouse brain tissue, mouse spleen tissue, mouse spleen cells. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:100-1:500 1:1,000 |
| Uniprot #: | SwissProt: P0CW02 Mouse | P0CW03 Mouse |
| Alternative names: | Ly 6c Ly6c protein Ly6c1 Ly6c2 Lymphocyte antigen 6 complex Lymphocyte antigen 6 complex locus C Lymphocyte antigen 6C Lymphocyte antigen 6C1 Lymphocyte antigen 6C2 Lymphocyte antigen Ly 6C |
Images
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Fig1:
Western blot analysis of Ly6c on different lysates with Rabbit anti-Ly6c antibody (HA500088) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse testis tissue lysate (low expression) Lane 4: Mouse liver tissue lysate (low expression) Lane 5: Mouse kidney tissue lysate Lane 6: Mouse heart tissue lysate Lane 7: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500088) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Ly6c antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500088, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Ly6c antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500088, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Ly6c antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500088, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of mouse spleen cells labeling Ly6c (HA500088) and CD8-PE. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500088, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Compared with Rabbit IgG Isotype Control (iFluor™ 488, 1/1,000). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.