GABRB3 Rabbit Polyclonal Antibody
cat.: HA500090
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GABRB3 aa 400-443 (Cytoplasmic domain). |
| Positive control: | Rat brain tissue lysate, rat cerebellum tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, HepG2 cell lysate, 293 cell lysate, rat brain tissue, human lung tissue, mouse brain tissue. |
| Subcellular location: | Plasma membrane, Cell membrane, Cytoplasmic vesicle membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P28472 Human | P63080 Mouse | P63079 Rat |
| Alternative names: | ECA5 GABA alpha receptor beta-2 subunit GABA(A) receptor subunit beta-3 GABAA receptor beta 3 subunit GABAA receptor subunit beta 3 GABR B3 Gabrb3 Gamma aminobutyric acid (GABA) A receptor beta 3 Gamma aminobutyric acid receptor subunit beta 3 Gamma-aminobutyric acid receptor subunit beta-3 GBRB3_HUMAN MGC9051 |
Images
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Fig1:
Western blot analysis of GABRB3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500090, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Rat cerebellum tissue lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse cerebellum tissue lysate Lane 5: HepG2 cell lysate Lane 6: 293 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500090, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500090, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500090, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.