AKT2 Rabbit Polyclonal Antibody
cat.: HA500091
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human AKT2. |
| Positive control: | HEK-293 cell lysate, rat brain tissue lysate, mouse pancreas tissue lysate, rat brain tissue, rat kidney tissue, mouse testis tissue. |
| Subcellular location: | Nucleus, Cell membrane, Early endosome, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: P31751 Human | Q60823 Mouse | P47197 Rat |
| Alternative names: | Akt2 AKT2_HUMAN HIHGHH Murine thymoma viral (v-akt) homolog 2 murine thymoma viral (v-akt) homolog-2 Oncogene AKT2 protein kinase B beta PKB PKB beta PKBB PKBBETA PRKBB Protein kinase Akt 2 Protein kinase Akt-2 Protein kinase B beta RAC beta rac protein kinase beta RAC-BETA RAC-beta serine/threonine-protein kinase RAC-PK-beta RACbeta v akt murine thymoma viral oncogene homolog 2 V-AKT murine thymoma viral oncogene homolog 2 |
Images
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Fig1:
Western blot analysis of AKT2 on different lysates with Rabbit anti-AKT2 antibody (HA500091) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate(20 µg/Lane) Lane 2: Rat brain tissue lysate(40 µg/Lane) Lane 3: Mouse pancreas tissue lysate(40 µg/Lane) Exposure time: 24 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500091, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 56 kDa Observed band size: 56 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AKT2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500091, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-AKT2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500091, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-AKT2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500091, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.