AMPS Rabbit Polyclonal Antibody
cat.: HA500092
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human AMPS. |
| Positive control: | Jurkatcell lysate, NIH/3T3 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, mouse brain tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: P30566 Human | P54822 Mouse |
| Alternative names: | Adenylosuccinase Adenylosuccinate lyase ADSL AMPS ASase ASL OTTHUMP00000199172 OTTHUMP00000199173 PUR8_HUMAN |
Images
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Fig1:
Western blot analysis of AMPS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500092, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkatcell lysate Lane 2: NIH/3T3 cell lysate Lane 3: Mouse skeletal muscle tissue lysate Lane 4: Rat skeletal muscle tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-AMPS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500092, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.