DDX3 Rabbit Polyclonal Antibody
cat.: HA500093
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 73 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DDX3 aa 220-420. |
| Positive control: | NIH/3T3 cell lysate, K562 cell lysate, A431 cell lysate, HepG2 cell lysate, Jurkat cell lysate, rat testis tissue lysate, A549, LOVO. |
| Subcellular location: | Inflammasome, Centrosome, Cell membrane, Nucleus, Cytoplasm, Stress granule, Lamellipodium. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: O00571 Human | Q62167 Mouse |
| Alternative names: | ATP dependent RNA helicase DDX3X ATP-dependent RNA helicase DDX3X CAP Rf DBX DDX14 DDX3X DDX3X_HUMAN DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked DEAD box DEAD box protein 3 DEAD box protein 3 X-chromosomal DEAD box X isoform DEAD box, X isoform DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 DEAD/H box 3 DEAD/H box 3, X-linked Helicase like protein 2 Helicase-like protein 2 HLP2 X isoform X-chromosomal |
Images
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Fig1:
Western blot analysis of DDX3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500093, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: K562 cell lysate Lane 3: A431 cell lysate Lane 4: HepG2 cell lysate Lane 5: Jurkat cell lysate Lane 6: Rat testis tissue lysate |
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Fig2: ICC staining of DDX3 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500093, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of DDX3 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500093, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.