RAD52 Rabbit Polyclonal Antibody
cat.: HA500094
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RAD52 aa 390-418. |
| Positive control: | Hela cell lysate, PC-3M cell lysate, Daudi cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P43351 Human |
| Alternative names: | DNA repair protein RAD52 DNA repair protein RAD52 DNA repair protein RAD52 homolog RAD 52 Rad52 RAD52 homolog (S. cerevisiae) RAD52 homolog (S. cerevisiae) RAD52 homolog RAD52_HUMAN Recombination protein RAD52 Recombination protein RAD52 Rhabdomyosarcoma antigen MU RMS 40.23 |
Images
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Fig1:
Western blot analysis of RAD52 on different lysates with Rabbit anti-RAD52 antibody (HA500094) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500094) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.