S6K1 Rabbit Polyclonal Antibody
cat.: HA500095
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human S6K1 aa 346-525. |
| Positive control: | HeLa cell lysate, C6 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse thymus tissue lysate, mouse testis tissue. |
| Subcellular location: | Mitochondrion outer membrane, Mitochondrion, Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P23443 Human | Q8BSK8 Mouse | P67999 Rat |
| Alternative names: | 70 kDa ribosomal protein S6 kinase 1 KS6B1_HUMAN p70 alpha P70 beta 1 p70 ribosomal S6 kinase alpha p70 ribosomal S6 kinase beta 1 p70 S6 kinase alpha P70 S6 Kinase p70 S6 kinase, alpha 1 p70 S6 kinase, alpha 2 p70 S6K p70 S6K-alpha p70 S6KA p70(S6K) alpha p70(S6K)-alpha p70-alpha p70-S6K 1 p70-S6K P70S6K P70S6K1 p70S6Kb PS6K Ribosomal protein S6 kinase 70kDa polypeptide 1 Ribosomal protein S6 kinase beta 1 Ribosomal protein S6 kinase beta-1 Ribosomal protein S6 kinase I RPS6KB1 S6K S6K-beta-1 S6K1 Serine/threonine kinase 14 alpha Serine/threonine-protein kinase 14A STK14A |
Images
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Fig1:
Western blot analysis of S6K1 on different lysates with Rabbit anti-S6K1 antibody (HA500095) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: C6 cell lysate Lane 3: PC-12 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Mouse thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500095) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-S6K1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500095, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.