ATG14L Rabbit Polyclonal Antibody
cat.: HA500096
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ATG14L aa 421-456. |
| Positive control: | HeLa cell lysates, rat brain tissue, human colon tissue, mouse testis tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Cytoplasm, Preautophagosomal structure membrane, autophagosome membrane. |
| Recommended Dilutions:
WB IHC-P |
1:4,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q6ZNE5 Human | Q8CDJ3 Mouse | D4A4K3 Rat |
| Alternative names: | 4832427M01 ATG14 Atg14L Autophagy-related protein 14-like protein BAKOR_HUMAN Barkor Beclin 1-associated autophagy-related key regulator D14Ertd114e D14Ertd436e KIAA0831 mCG_6911 |
Images
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Fig1:
Western blot analysis of ATG14L on HeLa cell lysate with Rabbit anti-ATG14L antibody (HA500096) at 1/4,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 40 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500096, 1/4,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 55 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATG14L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500096, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ATG14L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500096, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATG14L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500096, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.