HA500097

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Rabbit, Pig, Cow, Dog
Applications:	WB, IHC-P, IF
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage buffer:

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

Predicted band size: 136 kDa.

Isotype:

IgG

Immunogen:

KLH conjugated synthetic peptide derived from human PER1 protein 751-850/1290.

Positive control:

Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle. Low level in kidney.

Subcellular location:

Nucleus (By similarity). Cytoplasm (By similarity). Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2 (By similarity).

Recommended Dilutions:
  WB
  IHC-P
  IF

1:500-1:2,000
1:100-1:500
1:100-500

Uniprot #:

SwissProt: O15534 Human | O35973 Mouse | Q8CHI5 Rat

Alternative names:

Circadian clock protein PERIOD 1 Circadian clock protein PERIOD1 Circadian pacemaker protein Rigui hPER 1 hPER hPER1 KIAA0482 MGC88021 PER 1 PER PER1 PER1 protein PER1_HUMAN Period 1 Period circadian protein homolog 1 Period drosophila homolog of Period homolog 1 Period1 RIGUI

	Fig1: Sample: Lane 1: Human HeLa cell lysates Lane 2: Human A549 cell lysates Primary: Anti-PER1 (HA500097) at 1/1,000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution Predicted band size: 136 kDa Observed band size: 142 kDa
	Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-PER1 (HA500097) at 1/1,000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution Predicted band size: 136 kD Observed band size: 136 kD
	Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PER1) Polyclonal Antibody, Unconjugated (HA500097) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.