CD66b Rabbit Polyclonal Antibody
cat.: HA500100
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, mIHC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD66b aa 1-250. |
| Positive control: | U937 cell lysate, A431 cell lysate, Daudi cell lysate, human cervical cancer, human colon cancer tissue, human colon tissue. |
| Subcellular location: | Cell membrane, Cell surface. |
| Recommended Dilutions:
WB IHC-P mIHC |
1:500-1:1,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: P31997 Human |
| Alternative names: | Carcinoembryonic antigen CGM6 Carcinoembryonic antigen gene family member 6 Carcinoembryonic antigen related cell adhesion molecule 8 Carcinoembryonic antigen-related cell adhesion molecule 8 CD 66b CD 67 CD66b CD66b antigen CD67 CD67 antigen CEACAM 8 CEACAM8 CEAM8_HUMAN CGM 6 CGM6 NCA 95 NCA95 Non-specific cross-reacting antigen NCA-95 Nonspecific cross reacting antigen NCA 95 Nonspecific cross reacting antigen NCA95 |
Images
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Fig1:
Western blot analysis of CD66b on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500100, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U937 cell lysate Lane 2: A431 cell lysate Lane 2: Daudi cell lysate |
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Fig2: Fluorescence multiplex immunohistochemical analysis of the human cervical cancer (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD57 (HA601114, red), anti-CD11c (ET1606-19, green), anti-CD117 (HA21154, magenta) and anti-CD66b (HA500100, yellow) on human cervical cancer. Panel B: anti- CD57 stained on NKT cells. Panel C: anti-CD11c stained on dendritic cells. Panel D: anti-CD117 stained on mast cells. Panel E: anti-CD66b stained on neutrophils. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in four rounds of staining: in the order of HA601114 (1/500 dilution), ET1606-19 (1/1,000 dilution), HA721154 (1/1,000 dilution), and HA500100 (1/1,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD66b antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500100, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CD66b antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500100, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.