PPP3CC Rabbit Polyclonal Antibody
cat.: HA500101
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PPP3CC aa 1-200. |
| Positive control: | HepG2 cell lysate, mouse hippocampus tissue lysate, HepG2, mouse testis tissue. |
| Subcellular location: | Cytosol, presynaptic cytosol, mitochondrion, calcineurin complex, cytoplasm, glutamatergic synapse. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:100 1:100-1:500 |
| Uniprot #: | SwissProt: P48454 Human | P48455 Mouse |
| Alternative names: | calcineurin A gamma CALCINEURIN A3 Calcineurin Calcineurin testis specific catalytic subunit Calmodulin dependent calcineurin A subunit gamma isoform Calmodulin-dependent calcineurin A subunit gamma isoform CALNA3 CAM PRP catalytic subunit CAM-PRP catalytic subunit CNA3 pp2b gamma Antibody PP2BC_HUMAN PP2Bgamma Antibody PPP3CC protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform (calcineurin A gamma) protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform testis-specific catalytic subunit |
Images
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Fig1:
Western blot analysis of PPP3CC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500101, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Mouse hippocampus tissue lysate |
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Fig2: ICC staining of PPP3CC in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500101, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PPP3CC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500101, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.