UAP56 Rabbit Polyclonal Antibody
cat.: HA500102
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 49 kDa
Isotype: IgG
Immunogen: Recombinant protein within human UAP56 aa 100-300.
Positive control: 293 cell lysate, Hela cell lysate, rat bladder tissue, rat kidney tissue, human breast tissue, human esophagus tissue, mouse testis tissue.
Subcellular location: Cytoplasm, Nucleus, Spliceosome.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:1,000
1:100-1:500
Uniprot #: SwissProt: Q13838 Human | Q9Z1N5 Mouse | Q63413 Rat
Alternative names: 0610030D10Rik 4F2-LC6 56 kDa U2AF65-associated protein AI428441 ATP-dependent RNA helicase p47 B(0,+)-type amino acid transporter 1 BAT1 Bat1a D17H6S81E D17H6S81E-1 D6S81E D6S81Eh DDX39B DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B DEAD box protein UAP56 DX39B_HUMAN EC 3.6.1.- Glycoprotein-associated amino acid transporter b0,+AT1 HLA-B-associated transcript 1 protein HLA-B-associated transcript 1A HLA-B-associated transcript-1 MGC127051 MGC19235 MGC38799 nuclear RNA helicase (DEAD family) OTTHUMP00000029229 OTTHUMP00000165889 OTTHUMP00000165890 p47 Solute carrier family 7 member 9 Spliceosome RNA helicase BAT1 Spliceosome RNA helicase DDX39B U2AF65-associayed protein, 56-KD UAP56
Images
HA500102_1.jpg Fig1: Western blot analysis of UAP56 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500102, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: Hela cell lysate
HA500102_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-UAP56 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500102, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500102_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-UAP56 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500102, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500102_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-UAP56 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500102, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500102_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-UAP56 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500102, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500102_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UAP56 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500102, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.