TRIM25 Rabbit Polyclonal Antibody
cat.: HA500104
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRIM25 aa 20-320/630. |
| Positive control: | Jurkat cell lysate, 293 cell lysate, SK-Br-3 cell lysates, rat bladder tissue. |
| Subcellular location: | Cytoplasm, Stress granule. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q14258 Human |
| Alternative names: | E3 ubiquitin/ISG15 ligase TRIM25 EFP Estrogen responsive finger protein Estrogen-responsive finger protein RING finger protein 147 RNF 147 RNF147 TRI25 TRI25_HUMAN TRIM 25 Trim25 Tripartite motif containing 25 Tripartite motif containing protein 25 Tripartite motif protein TRIM25 Tripartite motif-containing protein 25 Tripartite motifcontaining25 Ubiquitin/ISG15-conjugating enzyme TRIM25 Z147 Zinc finger protein 147 (estrogen responsive finger protein) Zinc finger protein 147 Zinc finger protein-147 ZNF 147 ZNF147 |
Images
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Fig1:
Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody (HA500104) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si TRIM25 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500104) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TRIM25 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500104, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: 293 cell lysate |
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Fig3: Western blot analysis of TRIM25 on SK-Br-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500104, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-TRIM25 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500104, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.