TRAP alpha Rabbit Polyclonal Antibody
cat.: HA500105
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 32 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRAP alpha aa 19-200. |
| Positive control: | A431 cell lysate, HepG2 cell lysate, rat stomach tissue lysate, rat kidney tissue, mouse kidney tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: P43307 Human | Q9CY50 Mouse | Q7TPJ0 Rat |
| Alternative names: | DKFZp781N23103 FLJ14232 FLJ22100 FLJ23034 FLJ78242 FLJ93042 PGP35 Signal sequence receptor alpha Signal sequence receptor alpha subunit Signal sequence receptor subunit alpha SSR 1 SSR alpha SSR-alpha SSR1 SSRA_HUMAN SSRalpha Translocon associated protein alpha Translocon associated protein alpha subunit Translocon associated protein subunit alpha Translocon-associated protein subunit alpha TRAP a TRAP-alpha TRAPa TRAPalpha |
Images
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Fig1:
Western blot analysis of TRAP alpha on different lysates with Rabbit anti-TRAP alpha antibody (HA500105) at 1/5,000 dilution. Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 2: HL-60 (Human acute promyelocytic leukemia cell) cell lysate Lane 3: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 4: Rat stomach tissue lysate Lysates/proteins at 10 µg/Lane. Exposure time: 127 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500105, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 32 kDa Observed band size: 34 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-TRAP alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500105, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRAP alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500105, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.