IGF1 (E peptide) Rabbit Polyclonal Antibody
cat.: HA500107
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IGF1 aa 130-170. |
| Positive control: | Hela cell lysate, SW480 cell lysate, human liver tissue, K562. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:200-1:1000 1:50-1:100 |
| Uniprot #: | SwissProt: P05019 Human |
| Alternative names: | IBP1 IGF I IGF IA IGF IB IGF-I Igf1 IGF1_HUMAN IGF1A IGFI IGFIA Insulin like growth factor 1 (somatomedin C) Insulin like growth factor 1 Insulin like growth factor IA Insulin like growth factor IB Insulin-like growth factor I Mechano growth factor MGF OTTHUMP00000195080 OTTHUMP00000195081 OTTHUMP00000195082 OTTHUMP00000195083 OTTHUMP00000195084 Somatomedia C Somatomedin C Somatomedin-C |
Images
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Fig1:
Western blot analysis of IGF1 (E peptide) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500107, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SW480 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IGF1 (E peptide) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500107, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of IGF1 (E peptide) was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500107, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.