SH3PX1 Rabbit Polyclonal Antibody
cat.: HA500109
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 67 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SH3PX1 aa 430-595. |
| Positive control: | Hela cell lysate, 293T cell lysate, NIH/3T3 cell lysate, mouse heart tissue lysate, rat smooth muscle tissue lysate, rat uterus tissue, human placenta tissue, mouse testis tissue, Hela. |
| Subcellular location: | Trans-Golgi network, Cell membrane, Cytoplasmic vesicle membrane, Clathrin-coated vesicle, Ruffle, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9Y5X1 Human | Q91VH2 Mouse |
| Alternative names: | MST 155 MST155 MSTP 155 MSTP155 OTTHUMP00000040083 Protein SDP 1 Protein SDP1 SDP 1 SDP1 SH3 and PX domain containing protein 3A SH3 and PX domain containing protein SH3PX1 SH3 and PX domain-containing protein 1 SH3 and PX domain-containing protein 3A SH3PX1 SH3PXD3A SNX 9 SNX9 SNX9_HUMAN Sorting nexin 9 Sorting nexin-9 WASP interactor protein Wiskott Aldrich syndrome protein (WASP) interactor protein Wiskott Aldrich syndrome protein interactor protein WISP |
Images
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Fig1:
Western blot analysis of SH3PX1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500109, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Mouse heart tissue lysate Lane 5: Rat smooth muscle tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-SH3PX1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500109, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SH3PX1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500109, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SH3PX1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500109, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of Hela cells labeling SH3PX1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500109, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.