| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GXYLT1 aa 400-440. |
| Positive control: | U-937 cell lysate, HL-60 cell lysate, HUVEC cell lysate, LNCaP cell lysate, HepG2 cell lysate, HeLa cell lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q4G148 Human |
| Alternative names: | Glycosyltransferase 8 domain-containing protein 3 Glucoside xylosyltransferase 1 GXYLT1 GLT8D3 |
|
Fig1:
Western blot analysis of GXYLT1 on different lysates with Rabbit anti-GXYLT1 antibody (HA500113) at 1/5,000 dilution. Lane 1: U-937 cell lysate Lane 2: HL-60 cell lysate Lane 3: HUVEC cell lysate Lane 4: LNCaP cell lysate Lane 5: HepG2 cell lysate Lane 6: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500113) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |