GXYLT1 Rabbit Polyclonal Antibody
cat.: HA500113
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 51 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within human GXYLT1 aa 400-440.
Positive control: U-937 cell lysate, HL-60 cell lysate, HUVEC cell lysate, LNCaP cell lysate, HepG2 cell lysate, HeLa cell lysate.
Subcellular location: Membrane.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: Q4G148 Human
Alternative names: Glycosyltransferase 8 domain-containing protein 3 Glucoside xylosyltransferase 1 GXYLT1 GLT8D3
Images
HA500113_1.jpg Fig1: Western blot analysis of GXYLT1 on different lysates with Rabbit anti-GXYLT1 antibody (HA500113) at 1/5,000 dilution.

Lane 1: U-937 cell lysate
Lane 2: HL-60 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: LNCaP cell lysate
Lane 5: HepG2 cell lysate
Lane 6: HeLa cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500113) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.