Phospho-AKT2 (S474) Rabbit Polyclonal Antibody
cat.: HA500116
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AKT2 aa 450-481(phospho S474). |
| Positive control: | Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate, human kidney tissue, mouse testis tissue. |
| Subcellular location: | Cell membrane, Nucleus, Early endosome, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P31751 Human | Q60823 Mouse |
| Alternative names: | Akt2 AKT2_HUMAN HIHGHH Murine thymoma viral (v-akt) homolog 2 murine thymoma viral (v-akt) homolog-2 Oncogene AKT2 protein kinase B beta PKB PKB beta PKBB PKBBETA PRKBB Protein kinase Akt 2 Protein kinase Akt-2 Protein kinase B beta RAC beta rac protein kinase beta RAC-BETA RAC-beta serine/threonine-protein kinase RAC-PK-beta RACbeta v akt murine thymoma viral oncogene homolog 2 V-AKT murine thymoma viral oncogene homolog 2 |
Images
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Fig1:
Western blot analysis of Phospho-AKT2 (S474) on different lysates with Rabbit anti-Phospho-AKT2 (S474) antibody (HA500116) at 1/5,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: HEK-293 (Human embryonic kidney cell) cell lysate Lane 4: HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500116, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 56 kDa Observed band size: 56 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-AKT2 (S474) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500116, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-AKT2 (S474) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500116, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.