Phospho-AKT2 (S474) Rabbit Polyclonal Antibody
cat.: HA500116
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AKT2 aa 450-481(phospho S474). |
| Positive control: | Hela treated with Calyculin A whole cell lysate, Hela treated with insulin whole cell lysate, human kidney tissue, mouse testis tissue. |
| Subcellular location: | Cell membrane, Nucleus, Early endosome, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P31751 Human | Q60823 Mouse |
| Alternative names: | Akt2 AKT2_HUMAN HIHGHH Murine thymoma viral (v-akt) homolog 2 murine thymoma viral (v-akt) homolog-2 Oncogene AKT2 protein kinase B beta PKB PKB beta PKBB PKBBETA PRKBB Protein kinase Akt 2 Protein kinase Akt-2 Protein kinase B beta RAC beta rac protein kinase beta RAC-BETA RAC-beta serine/threonine-protein kinase RAC-PK-beta RACbeta v akt murine thymoma viral oncogene homolog 2 V-AKT murine thymoma viral oncogene homolog 2 |
Images
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Fig1:
Western blot analysis of Phospho-AKT2 (S474) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500116, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated Hela whole cell lysates Lane 2: Hela treated with Calyculin A whole cell lysates Lane 3: Hela treated with insulin whole cell lysates |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-AKT2 (S474) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500116, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-AKT2 (S474) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500116, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.