Activin Receptor Type IA Rabbit Polyclonal Antibody
cat.: HA500117
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Activin Receptor Type IA aa 440-480. |
| Positive control: | Mouse skeletal muscle tissue lysate, PC-3 cell lysate, rat skeletal muscle tissue lysate, MG-63. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q04771 Human | P37172 Mouse | P80201 Rat |
| Alternative names: | Activin A receptor type I Activin A receptor type II like kinase 2 Activin receptor type I Activin receptor type-1 Activin receptor-like kinase 2 ACTR-I ACTRI Acvr1 ACVR1_HUMAN ACVR1A ACVRLK2 ALK 2 ALK-2 ALK2 FOP Hydroxyalkyl protein kinase Serine/threonine-protein kinase receptor R1 SKR1 TGF-B superfamily receptor type I TGFB superfamily receptor type I TSR-I TSRI |
Images
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Fig1:
Western blot analysis of Activin Receptor Type IA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500117, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse skeletal muscle tissue lysate Lane 2: PC-3 cell lysate Lane 3: Rat skeletal muscle tissue lysate |
|
Fig2: ICC staining of Activin Receptor Type IA in MG-63 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500117, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.