| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SCD1 aa 1-50. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, Mouse liver tissue lysate, Mouse white adipose tissue lysate, Rat white adipose tissue lysate. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: O00767 Human | P13516 Mouse | P07308 Rat |
| Alternative names: | ACOD_HUMAN Acyl-CoA desaturase Delta(9)-desaturase Delta-9 desaturase Delta-9-Desaturase FADS5 Fatty acid desaturase PRO0998 SCD 1 SCD SCD1 Stearoyl-CoA desaturase |
|
Fig1:
Western blot analysis of SCD1 on different lysates with Rabbit anti-SCD1 antibody (HA500119) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Mouse white adipose tissue lysate (40 µg/Lane) Lane 5: Rat white adipose tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 37 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500119) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |