SCD1 Rabbit Polyclonal Antibody
cat.: HA500119
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 42 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human SCD1 aa 1-50.
Positive control: HeLa cell lysate, HepG2 cell lysate, Mouse liver tissue lysate, Mouse white adipose tissue lysate, Rat white adipose tissue lysate.
Subcellular location: Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: O00767 Human | P13516 Mouse | P07308 Rat
Alternative names: ACOD_HUMAN Acyl-CoA desaturase Delta(9)-desaturase Delta-9 desaturase Delta-9-Desaturase FADS5 Fatty acid desaturase PRO0998 SCD 1 SCD SCD1 Stearoyl-CoA desaturase
Images
HA500119_1.jpg Fig1: Western blot analysis of SCD1 on different lysates with Rabbit anti-SCD1 antibody (HA500119) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Mouse white adipose tissue lysate (40 µg/Lane)
Lane 5: Rat white adipose tissue lysate (40 µg/Lane)

Predicted band size: 42 kDa
Observed band size: 37 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500119) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.