MCRS1 Rabbit Polyclonal Antibody
cat.: HA500120
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 52 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human MCRS1 aa 1-200.
Positive control: SH-SY5Y cell lysates, rat large intestine tissue, human liver tissue, human pancreas tissue, mouse testis tissue.
Subcellular location: Nucleus, Nucleolus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:2,000
1:100-1:500
Uniprot #: SwissProt: Q96EZ8 Human | Q99L90 Mouse | G3V9E0 Rat
Alternative names: 58 kDa microspherule protein Cell cycle regulated factor (78 kDa) Cell cycle regulated factor p78 Cell cycle-regulated factor p78 ICP22BP INO80 complex subunit J MCRS 1 MCRS 2 MCRS1 MCRS1_HUMAN MCRS2 Microspherule protein 1 MSP 58 MSP58 p78
Images
HA500120_1.jpg Fig1: Western blot analysis of MCRS1 on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500120, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.ne 2: SH-SY5Y cell lysate
HA500120_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MCRS1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500120, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500120_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MCRS1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500120, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500120_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MCRS1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500120, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500120_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MCRS1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500120, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.