FAM13C1 Rabbit Polyclonal Antibody
cat.: HA500122
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 66 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human FAM13C1 aa 241-270.
Positive control: HepG2 cell lysate, rat brain tissue lysate, rat large intestine tissue.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: Q8NE31 Human
Alternative names: Protein FAM13C FAM13C FAM13C1
Images
HA500122_1.jpg Fig1: Western blot analysis of FAM13C1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500122, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: Rat brain tissue lysate
HA500122_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-FAM13C1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500122, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.