PPP2R4 Rabbit Polyclonal Antibody
cat.: HA500123
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PPP2R4 aa 1-200. |
| Positive control: | MCF7 cell lysate, SK-Br-3 cell lysate, SiHa cell lysate, Mouse brain tissue lysate, human kidney tissue, rat bladder tissue, MCF7. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:2,000-1:10,000 1:400-1:1,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q15257 Human | P58389 Mouse | B2RYQ2 Rat |
| Alternative names: | subunit B'' KIAA0044 MGC2184 Phosphotyrosyl phosphatase activator PP2A PP2A phosphatase activator PP2A subunit B' PP2A subunit B' isoform PR53 PP2A subunit B' PR53 isoform PPP2R4 PR53 PR53 isoform Protein phosphatase 2, regulatory subunit B-prime Protein phosphatase 2A activator regulatory subunit 4 Protein phosphatase 2A regulatory subunit B' (PR 53) PTPA PTPA_HUMAN Serine/threonine protein phosphatase 2A regulatory subunit B' Serine/threonine-protein phosphatase 2A activator Serine/threonine-protein phosphatase 2A regulatory subunit 4 Serine/threonine-protein phosphatase 2A regulatory subunit B'' |
Images
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Fig1:
Western blot analysis of PPP2R4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500123, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF7 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: SiHa cell lysate Lane 4: Mouse brain tissue lysate |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PPP2R4 antibody (HA500123) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500123) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-PPP2R4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500123, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of MCF7 cells labeling PPP2R4 with Rabbit anti-PPP2R4 antibody (HA500123) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPP2R4 antibody (HA500123) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of MCF7 cells labeling PPP2R4. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500123, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.