mTOR Rabbit Polyclonal Antibody
cat.: HA500126
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 289 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human mTOR aa 2430-2480. |
| Positive control: | MCF-7 cell lysate, rat brain tissue lysate, HepG2 cell lysate, rat testis tissue, human breast carcinoma tissue, mouse heart tissue. |
| Subcellular location: | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Lysosome, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:200 1:100-1:500 |
| Uniprot #: | SwissProt: P42345 Human | Q9JLN9 Mouse | P42346 Rat |
| Alternative names: | dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) FK506 binding protein 12 rapamycin associated protein 1 FK506 binding protein 12 rapamycin associated protein 2 FK506 binding protein 12 rapamycin complex associated protein 1 FK506-binding protein 12-rapamycin complex-associated protein 1 FKBP rapamycin associated protein FKBP12 rapamycin complex associated protein FKBP12-rapamycin complex-associated protein 1 FKBP12-rapamycin complex-associated protein FLJ44809 FRAP FRAP1 FRAP2 Mammalian target of rapamycin Mechanistic target of rapamycin mTOR MTOR_HUMAN OTTHUMP00000001983 RAFT1 Rapamycin and FKBP12 target 1 Rapamycin associated protein FRAP2 Rapamycin target protein 1 Rapamycin target protein RAPT1 Serine/threonine-protein kinase mTOR |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-mTOR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500126, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-mTOR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500126, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-mTOR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500126, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of mTOR on different lysates with Rabbit anti-mTOR antibody (HA500126) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: PC-12 cell lysate Lane 5: C6 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500126, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 289 kDa Observed band size: 289 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.