GNB3 Rabbit Polyclonal Antibody
cat.: HA500127
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GNB3 aa 100-150. |
| Positive control: | HepG2 cell lysate, A549 cell lysate, mouse kidney tissue lysate, rat brian tissue lysate, F9, rat kidney tissue, mouse brian tissue. |
| Subcellular location: | Cytosol, Extracellular region or secreted, Plasma Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P16520 Human | Q61011 Mouse | P52287 Rat |
| Alternative names: | G protein beta 3 subunit GBB3_HUMAN GNB3 GNB3 guanine nucleotide binding protein (G protein), beta polypeptide 3 GTP binding regulatory protein beta 3 chain Guanine nucleotide binding protein (G protein) beta polypeptide 3 Guanine nucleotide binding protein G Guanine nucleotide binding protein G(I)/G(S)/G(T) beta subunit 3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 Hypertension associated protein IG SG Transducin beta chain 3 Tsubunit beta 3 |
Images
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Fig1: ICC staining of GNB3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500127, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GNB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500127, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brian tissue using anti-GNB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500127, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of GNB3 on different lysates with Rabbit anti-GNB3 antibody (HA500127) at 1/5,000 dilution. Lane 1: C6 cell lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500127, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37 kDa Observed band size: 37 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.