Placental lactogen Rabbit Polyclonal Antibody
cat.: HA500128
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Placental lactogen aa 50-217. |
| Positive control: | Human placenta tissue lysates, human placenta tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P0DML3 Human |
| Alternative names: | Choriomammotropin Chorionic somatomammotropin A chorionic somatomammotropin B chorionic somatomammotropin hormone 1 (placental lactogen) Chorionic somatomammotropin hormone 1 chorionic somatomammotropin hormone 2 Chorionic somatomammotropin hormone CS 2 CS-2 CS1 CSA CSB CSH_HUMAN CSH1 CSH2 CSMT hCS B hCS-B hCSA Lactogen mPL II PL PL1 Placental lactogen Prl3b1 prolactin family 3, subfamily b, member 1 |
Images
|
Fig1: Western blot analysis of Placental lactogen on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500128, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Placental lactogen antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500128, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.