THOC5 Rabbit Polyclonal Antibody
cat.: HA500129
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 79 kDa
Isotype: IgG
Immunogen: Recombinant protein within human THOC5 aa 1-200.
Positive control: NIH/3T3 cell lysate, PC-12 cell lysate, Hela cell lysate, HepG2 cell lysate, human ovarian tumor tissue, mouse brain tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:2,000
1:200-1:1,000
Uniprot #: SwissProt: Q13769 Human | Q8BKT7 Mouse | Q68FX7 Rat
Alternative names: C22orf19 Chromosome 22 open reading frame 19 Fmip Fms interacting protein fSAP79 Functional spliceosome-associated protein 79 hTREX90 KIAA0983 LOC400922 NF2/meningioma region protein pK1.3 PK1.3 Placental protein 39.2 PP39.2 THO complex 5 THO complex subunit 5 THO complex subunit 5 homolog thoc5 THOC5_HUMAN
Images
HA500129_1.jpg Fig1: Western blot analysis of THOC5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500129, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: HepG2 cell lysate
HA500129_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue using anti-THOC5 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500129, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500129_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-THOC5 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500129, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.