OC-3/2/1 Rabbit Polyclonal Antibody
cat.: HA500130
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ONECUT3 aa 430-470. |
| Positive control: | SW480 cell lysate, HeLa cell lysate, AGS cell lysate, SW620. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: O60422 Human | O95948 Human | Q9UBC0 Human |
| Alternative names: | OC 3 OC-3 OC3 One cut domain family member 3 One cut homeobox 3 ONEC3_HUMAN ONECUT 3 Onecut3 Transcription factor ONECUT 3 Transcription factor ONECUT-3 Transcription factor ONECUT3 |
Images
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Fig1:
Western blot analysis of OC-3/2/1 on different lysates with Rabbit anti-OC-3/2/1 antibody (HA500130) at 1/2,000 dilution. Lane 1: SW480 cell lysate Lane 2: HeLa cell lysate Lane 3: AGS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500130) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of OC-3/2/1 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500130, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.