Beta-galactosidase Rabbit Polyclonal Antibody
cat.: HA500132
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Beta-galactosidase aa 20-60. |
| Positive control: | MCF-7 cell lysate, mouse pancreas tissue lysate, rat stomach tissue lysate, PANC-1, rat kidney tissue, human liver tissue, mouse testis tissue. |
| Subcellular location: | Lysosome, perinuclear region. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:100 1:200-1:1,000 |
| Uniprot #: | SwissProt: P16278 Human | P23780 Mouse |
| Alternative names: | Acid beta galactosidase Acid beta-galactosidase Beta galactosidase 1 Beta galactosidase Beta-galactosidase BGAL_HUMAN EBP EBP, included Elastin receptor 1 (67kD) Elastin receptor 1 67kDa Elastin receptor 1 Elastin receptor 1, included Elastin-binding protein, included ELNR1 Galactosidase beta 1 GLB 1 GLB1 Lactase MPS4B S-GAL, included |
Images
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Fig1:
Western blot analysis of Beta-galactosidase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500132, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Mouse pancreas tissue lysate Lane 3: Rat stomach tissue lysate |
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Fig2: ICC staining of Beta-galactosidase in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500132, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Beta-galactosidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500132, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Beta-galactosidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500132, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Beta-galactosidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500132, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.