GNAO1 Rabbit Polyclonal Antibody
cat.: HA500136
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GNAO1 aa 1-200. |
| Positive control: | Rat brain tissue lysate, mouse brain tissue lysate, mouse hippocampus tissue lysate, human brain tissue lysate, human pancreas tissue. |
| Subcellular location: | Cell membrane, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000-1:10,000 1:100-1:500 |
| Uniprot #: | SwissProt: P09471 Human | P18872 Mouse | P59215 Rat |
| Alternative names: | DKFZp686O0962 G alpha o G protein alpha 0 Galphao GNAO 1 GNAO GNAO_HUMAN GNAO1 Go alpha Guanine nucleotide binding protein (G protein) alpha activating activity polypeptide O Guanine nucleotide binding protein alpha activating polypeptide Guanine nucleotide binding protein G(o) subunit alpha Guanine nucleotide-binding protein G(o) subunit alpha |
Images
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Fig1:
Western blot analysis of GNAO1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500136, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Human brain tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GNAO1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500136, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.