GNAO1 Rabbit Polyclonal Antibody
cat.: HA500136
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GNAO1 aa 1-200. |
| Positive control: | Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cell membrane, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:20,000 1:2,000 |
| Uniprot #: | SwissProt: P09471 Human | P18872 Mouse | P59215 Rat |
| Alternative names: | DKFZp686O0962 G alpha o G protein alpha 0 Galphao GNAO 1 GNAO GNAO_HUMAN GNAO1 Go alpha Guanine nucleotide binding protein (G protein) alpha activating activity polypeptide O Guanine nucleotide binding protein alpha activating polypeptide Guanine nucleotide binding protein G(o) subunit alpha Guanine nucleotide-binding protein G(o) subunit alpha |
Images
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Fig1:
Western blot analysis of GNAO1 on different lysates with Rabbit anti-GNAO1 antibody (HA500136) at 1/20,000 dilution. Lane 1: Mouse brain tissue lysate (10 µg/Lane) Lane 2: Mouse cerebellum tissue lysate (10 µg/Lane) Lane 3: Rat brain tissue lysate (10 µg/Lane) Lane 4: Rat cerebellum tissue lysate (10 µg/Lane) Predicted band size: 40 kDa Observed band size: 37 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500136) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GNAO1 antibody (HA500136) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500136) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GNAO1 antibody (HA500136) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500136) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GNAO1 antibody (HA500136) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500136) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.