Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 37 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human PP1C gamma aa 293-323. |
Positive control: | 293 cell lysate, mouse lung tissue lysate, rat brain tissue lysate, human prostate tissue, human stomach tissue. |
Subcellular location: | Nucleus, Mitochondrion, Cytoplasm, Kinetochore, Cleavage furrow, Midbody. |
Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:100-1:500 |
Uniprot #: | SwissProt: P36873 Human | P63087 Mouse | P63088 Rat |
Alternative names: | PP 1G PP-1G PP1C PP1G PP1G_HUMAN PP1gamma PPP 1G PPP1CC PPP1G Protein phosphatase 1, catalytic subunit, gamma isozyme Protein phosphatase 1C catalytic subunit Serine/threonine phosphatase 1 gamma Serine/threonine protein phosphatase PP1 gamma catalytic subunit Serine/threonine-protein phosphatase PP1-gamma catalytic subunit |
Fig1:
Western blot analysis of PP1C gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500138, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: Mouse lung tissue lysate Lane 3: Rat brain tissue lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-PP1C gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500138, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-PP1C gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500138, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |