| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PIP5K2 gamma aa 100-300. |
| Positive control: | MCF-7 cell lysate, A549 cell lysate, rat cerebellum tissue lysate, mouse brain tissue lysate. |
| Subcellular location: | Cytoplasm, Endoplasmic reticulum. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q8TBX8 Human | Q91XU3 Mouse | O88370 Rat |
| Alternative names: | Phosphatidylinositol 4 phosphate 5 kinase Phosphatidylinositol 4 phosphate 5 kinase type II gamma Phosphatidylinositol-5-phosphate 4-kinase type II gamma Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma PI(5)P 4-kinase type II gamma PI42C_HUMAN Pip4k2c PIP4KII-gamma PIP5K2C |
|
Fig1:
Western blot analysis of PIP5K2 gamma on different lysates with Rabbit anti-PIP5K2 gamma antibody (HA500139) at 1/1,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500139, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 47.3 kDa Observed band size: 47 kDa |