TNF Receptor I Rabbit Polyclonal Antibody
cat.: HA500140
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TNF Receptor I aa 233-455. |
| Positive control: | A549 cell lysate, Hela cell lysate, HepG2 cell lysate, human fallopian tube tissue, Hela. |
| Subcellular location: | Secreted, Cell membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:2,000 1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: P19438 Human |
| Alternative names: | CD120a FPF MGC19588 p55 p55-R p60 TBP1 TBPI TNF R TNF R55 TNF-R1 TNF-RI TNFAR TNFR-I TNFR1 TNFR55 TNFR60 TNFRI TNFRSF1a TNR1A_HUMAN Tumor necrosis factor receptor 1 Tumor necrosis factor receptor superfamily, member 1A Tumor necrosis factor receptor type 1 Tumor necrosis factor receptor type I Tumor necrosis factor-binding protein 1 |
Images
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Fig1:
Western blot analysis of TNF Receptor I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500140, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using anti-TNF Receptor I antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500140, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of Hela cells labeling TNF Receptor I. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500140, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.