MCK10 Rabbit Polyclonal Antibody
cat.: HA500141
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 101 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MCK10 aa 150-417. |
| Positive control: | Mouse kidney tissue, rat kidney tissue, Hela, T-47D cell lysate, SK-Br-3 cell lysate. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:5,000 1:100-1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q03146 Mouse | Q63474 Rat | Q08345 Human |
| Alternative names: | CAK CD 167 CD167 CD167 antigen-like family member A CD167a Cell adhesion kinase DDR 1 DDR DDR1 DDR1_HUMAN Discoidin domain receptor Discoidin domain receptor tyrosine kinase 1 Discoidin receptor tyrosine kinase Discoidin receptor tyrosine kinase isoform a EDDR 1 EDDR1 Epithelial discoidin domain receptor 1 Epithelial discoidin domain-containing receptor 1 Epithelial specific receptor kinase HGK2 Mammarian carcinoma kinase 10 Mammary carcinoma kinase 10 MCK-10 MCK10 NEP Neuroepithelial tyrosine kinase Neurotrophic tyrosine kinase receptor type 4 NTRK 4 NTRK4 OTTHUMP00000029343 OTTHUMP00000029344 OTTHUMP00000029345 OTTHUMP00000029346 OTTHUMP00000029347 OTTHUMP00000164863 OTTHUMP00000164867 OTTHUMP00000222080 Protein-tyrosine kinase 3A Protein-tyrosine kinase RTK-6 PTK 3 PTK 3A protein tyrosine kinase 3A PTK3 PTK3A Receptor tyrosine kinase NEP RTK 6 RTK6 TRK E TRKE Tyrosine kinase DDR Tyrosin...... |
Images
|
Fig1: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MCK10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500141, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-MCK10 antibody (HA500141) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500141) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of Hela cells labeling MCK10. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500141, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig4:
Western blot analysis of MCK10 on different lysates with Rabbit anti-MCK10 antibody (HA500141) at 1/5,000 dilution. Lane 1: T-47D cell lysate Lane 2: SK-Br-3 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500141, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 101 kDa Observed band size: 101 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.