LKB1 Rabbit Polyclonal Antibody
cat.: HA500143
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LKB1 aa 1-200. |
| Positive control: | Rat testis tissue lysate, human liverl tissue lysate, mouse liver tissue lysate, human liver tissue, rat testis tissue. |
| Subcellular location: | Cytoplasm, Membrane, Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q15831 Human | Q9WTK7 Mouse | D4AE59 Rat |
| Alternative names: | hLKB1 Liver kinase B1 LKB1 PJS Polarization related protein LKB1 Renal carcinoma antigen NY-REN-19 Serine/Threonine Kinase 11 Serine/threonine protein kinase 11 Serine/threonine protein kinase LKB1 Serine/threonine protein kinase STK11 Serine/threonine-protein kinase 11 Serine/threonine-protein kinase LKB1 Serine/threonine-protein kinase XEEK1 Stk11 STK11_HUMAN |
Images
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Fig1:
Western blot analysis of LKB1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500143, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat testis tissue lysate Lane 2: Human liverl tissue lysate Lane 3: Mouse liver tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LKB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500143, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-LKB1 antibody (HA500143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500143) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.