HNRPDL Rabbit Polyclonal Antibody
cat.: HA500146
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse HNRPDL aa 1-148. |
| Positive control: | Rat brain tissue, human colon carcinoma tissue, mouse kidney tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: O14979 Human | D3YTQ3 Mouse | Q9Z130 Mouse | Q3SWU3 Rat |
| Alternative names: | A+U-rich element RNA binding factor AA407431 AA959857 AU rich element RNA binding factor AU-rich element RNA-binding factor D5Ertd650e D5Wsu145e Heterogeneous nuclear ribonucleoprotein D like Heterogeneous nuclear ribonucleoprotein D like protein Heterogeneous nuclear ribonucleoprotein D-like hnHNRP DL HNRDL_HUMAN HNRNP hnRNP D-like hnRNP DL HNRNPDL hnRPD like protein JKT41 binding protein JKT41-binding protein JKTBP JKTBP2 laAUF1 MGC125262 Protein laAUF1 |
Images
|
Fig1:
Western blot analysis of HNRPDL on HNRPDL recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500146) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HNRPDL recombinant protein with primary antibody at 1:500 dilutions Lane 2: HNRPDL recombinant protein with primary antibody at 1:1,000 dilutions |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HNRPDL antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500146, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-HNRPDL antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500146, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HNRPDL antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500146, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.