XRCC2 Rabbit Polyclonal Antibody
cat.: HA500147
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human XRCC2 aa 1-200. |
| Positive control: | 293T (Human embryonic kidney cell) cell lysate, mouse testis tissue. |
| Subcellular location: | Nucleus, centrosome. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:5,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q9CX47 Mouse | O43543 Human |
| Alternative names: | DKFZp781P0919 DNA repair protein XRCC2 X ray repair complementing defective repair in Chinese hamster cells 2 X ray repair cross complementing protein 2 X-ray repair cross-complementing protein 2 Xrcc2 XRCC2_HUMAN |
Images
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Fig1:
Western blot analysis of XRCC2 on 293T (Human embryonic kidney cell) cell lysate with Rabbit anti-XRCC2 antibody (HA500076) at 1/5,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500076, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-XRCC2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500147, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.