FLI1 Rabbit Polyclonal Antibody
cat.: HA500149
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FLI1 aa 1-200. |
| Positive control: | NIH/3T3 cell lysate, Jurkat cell lysate, rat spleen tissue, human colon tissue, human spleen tissue, mouse spleen tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P WB |
1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q01543 Human | P26323 Mouse |
| Alternative names: | ERGB transcription factor Ewing Sarcoma breakpoint region 2 EWSR 2 EWSR2 FLI 1 FLI 1 proto oncogene Fli-1 proto-oncogene, ETS transcription factor FLI1 FLI1 EWS fusion gene FLI1 proto oncogene FLI1_HUMAN Friend leukemia integration 1 (FLI1) transcription factor Friend leukemia integration 1 transcription factor Friend leukemia virus integration 1 Proto-oncogene Fli-1 Sarcoma breakpoint region 2 (EWSR2) SIC 1 SIC1 Transcription factor ERGB Viral integration region FLI1 Viral integration region FLI1, mouse, homolog of |
Images
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Fig1:
Western blot analysis of FLI1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500149, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Jurkat cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-FLI1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500149, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-FLI1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500149, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FLI1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500149, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FLI1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500149, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.