FLI1 Rabbit Polyclonal Antibody
cat.: HA500149
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FLI1 aa 1-200. |
| Positive control: | Rat spleen tissue , human colon tissue, human spleen tissue, mouse spleen tissue, Jurkat cell lysate, MOLT-4 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P WB |
1:10,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q01543 Human | P26323 Mouse |
| Alternative names: | ERGB transcription factor Ewing Sarcoma breakpoint region 2 EWSR 2 EWSR2 FLI 1 FLI 1 proto oncogene Fli-1 proto-oncogene, ETS transcription factor FLI1 FLI1 EWS fusion gene FLI1 proto oncogene FLI1_HUMAN Friend leukemia integration 1 (FLI1) transcription factor Friend leukemia integration 1 transcription factor Friend leukemia virus integration 1 Proto-oncogene Fli-1 Sarcoma breakpoint region 2 (EWSR2) SIC 1 SIC1 Transcription factor ERGB Viral integration region FLI1 Viral integration region FLI1, mouse, homolog of |
Images
|
Fig1: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-FLI1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500149, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-FLI1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500149, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FLI1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500149, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FLI1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500149, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Western blot analysis of FLI1 on different lysates with Rabbit anti-FLI1 antibody (HA500149) at 1/10,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: MOLT-4 cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 14 seconds; ECL: K1801 Negative expression of FLI1 protein in SH-SY5Y cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500149, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 51 kDa Observed band size: 51 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.